This technique is widely used in laboratories, especially in those of molecular biology, because it is used in important procedures such as: separation, analysis and purification of RNA, DNA, or proteins, nucleic acids, this process is performed because most biomolecules have an electrical charge where their magnitude depends on the pH of the medium in which they are found; because of this, the biomolecules move when subjected to an electric field to the charge pole opposite to that of the molecule.
Its operation is a simple process, separates the molecules according to their size and electrical charge, for this an electric current is used that impels to displace the molecules through a gel or another matrix., then the pores of the gel act as a sieve, allowing the smaller molecules to move faster than the larger molecules, to know the size of the molecules in a sample, they are compared with the established size standards that are separated into the same gel.
In the case of proteins that can have a positive or negative charge, are usually neutral charge, for the migration of molecules pretreatment with detergents, such as sodium dodecyl sulfate (SDS), which confers negative charge; this homogenizes the proteins in the sample and will all migrate to the positive pole; will only be separated by size.
Now on the side of nucleic acids only have a negative charge, because of their phosphate skeleton, instead electrophoresis, causes nucleic acids to migrate towards the positive pole, called anode. This migration technique is what we call the principle of electrophoresis, where the displacement of the molecules through a gel or other type of porous matrix occurs proportionally, using the parameters of molecular weight or size; movement generated by the electric field.
YR horizontal gel electrophoresis units have been designed by scientists with the laboratory environment...
YR horizontal gel electrophoresis units have been designed by scientists with the laboratory environment ...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally ...
| Model | YR03433 |
| Gel Size(W×L)mm | 120×145 |
| Gel thickness | 1,1.5(mm) |
| Comb wells | 16, 23 |
| Gel capacity | 2 |
| Buffer requirement | 1200(ml) |
The electrophoresis chamber is the device where the sample is introduced for said process; and where the electromagnetic field that is formed in the electrophoresis...
Electrophoresis is a laboratory technique in which a controlled electric current is used in order to separate biomolecules according to their size and electric ...
Electrophoresis consists of a technique that allows the separation of biomolecules according to their mobility...
This technique is widely used in laboratories, especially in those of molecular biology, because it is used...
Electrophoresis is an analytical technique in which a controlled electric current is used in order to separate biomolecules...
Electrophoresis is one of the main molecular biology techniques, they are considered the most used technique in ...
To establish the identity of plant or animal species for food purposes, based only on the observation of external ...
Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA, and other proteins according to their respective molecular size and charge. Gel electrophoresis can be of two different methods: horizontal gel electrophoresis ...
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