A thermal cell works through three important steps during the PCR technique. The first step is denaturation, which occurs in the first cycle. From there, part to the following cycles to separate formed strands. While this occurs the temperature ranges between 94 and 98 º C. This process lasts at least 10 seconds.
The second step consists of the alignment of the firsts in the thread of the mold that you want to amplify. The passage lasts about 20 seconds and its temperature can become variable. This happens because it depends more than all of the firsts used in this operation. The firsts are small sequences of 20 nucleotides in which tests with simple sugars are used. On many occasions, glucose is part of it, the temperature used for this type of tests ranges between 55 and 65 º C, although some can reach up to 72 º C. This is the temperature suitable for working with Taq polymerase.
The third step is the elongation of the double strand of 20 pairs which has been created in the previous step. Elongation time should not be more than a minute by kilobase.