The Microplate reader is a device designed to perform diagnostic tests applied to the detection of infectious diseases that pose a threat to the individual, or to require treatments with epidemiological monitoring. This equipment is highly functional through the practice of ELISA, being one of the most reliable tests that are carried out in the laboratories.
From the characterization of immune responses in inoculated or infected people, to the location of the expression of a protein that is formulated in the cell extension, to execute quality control trials. And they are of great importance, since having teams able to make such assessments is the key.
Because there are many cases within science, the detection of viruses such as Hepatitis B, the use of Microplate readers will allow us to provide timely and cost-effective responses. And Kalstein has the ideal equipment for such measurements, thanks to its technological and digital power, it reaches to exceed the expectations of users within laboratory practices.
Importance of the Microplate Reader for detecting Hepatitis B
The Microplate reader is the equipment that uses the ELISA test, consists of sheets where contain wells where the samples rest to be studied, so during laboratory practices, the sample is placed to detect any infectious agent, such as the response to antibodies that cause the host, depending on the distribution of the test chosen. As a result, Microplate readers can identify chronically infected individuals, especially when the load of dangerous agents develops or decreases. However, sustained antibody collection in individuals can be achieved when the microbe itself cannot.
In this sense, hepatitis B is a highly infectious disease that affects the liver and can go asymptomatically to chronic processes, in which the health of the individual is compromised. Therefore, the ELISA method with the use of the Microplate Reader, is the direct immunoenzymatic method, and in practice towards the diagnosis is applied the type of technique type sandwich, where the wells of the equipment are coated with reagents that act as capture antibodies. The wells are covered with monoclonal antibodies for various subtypes of the virus. In the course of the study, the development of the practice, samples and antibodies attached to an enzyme are added to the wells and incubated. After incubation, the wells should be washed to remove any unbound residue or material, and automatically add the corresponding substrates and then incubate to form shades of blue, indicating the amount of virus present in the sample.
All this corresponds to the concentration of Hepatitis B present and is measured thanks to the Microplate reader at 450/630-700 or 450 nm.
This type of practice is performed qualitatively in human serum or plasma, where the prognosis of the test is monitoring and diagnostic aid in the case of possible infection by the Hepatitis B virus. And for this purpose should comply with the following recommendations and avoid accidents within the practices, such as:
- Only trained professional personnel perform the in vitro practice.
- Do not use after the expiry date.
- Avoid any contamination between the reagents.
- Perfectly wash wells ensuring performance for future testing.
- Pipette the samples with new tips.
- The surface of the Microplate should be clean and dry so that no wells contain air bubbles.
- Wearing gloves to avoid leaving traces.
- Calibrate your computer correctly.
Kalstein Brand Microplate Reader
Currently, in the market there are several medical and laboratory equipment, but none like ours in Kalstein, as manufacturers we offer our users the best technology for our customers to practice confidently for efficient results. We have the Microplate Readers, belonging to the YR series, with attractive features such as:
- 8-channel visual system, for quantitative and qualitative testing.
- Spectacular external jet printing system
- High-light LED long-life > 100,000 hours
- External computerized system, with a large storage capacity for 1000 test items and 1,000,000 results.
To learn more about our products, visit our catalog from HERE We are manufacturers of the best electronic equipment and we have the best advice to make your purchase is the most appropriate and at excellent prices.